Gingko Leaf

The SEM image shows a Gingko leaf. The sample was collected from the Botanical Gardens in Cambridge. A small section of the stem was cut from the leaf and mounted using a mixture of Tissue Tek and colloidal graphite (70:30 ratio) onto a cryo stub. The sample was then plunged freezed in LN2, fratured and coated with 5 nm of Au/Pd using the Quorum PP3010 cryo system. This sample was then loaded onto a Quorum cold stage and imaged within a Zeiss Evo. A beam energy of 6.00kV was used with a probe current of 16 pA.